Verification of insertions into universal mosSCI sites

I’ve had a few people ask me for sequences surrounding the universal mosSCI sites and oligos that can be used to verify insertions and homozygosity of the inserts. I have posted ApE files with the local genomic environment and the oligos we use to verify the position and homozygosity of inserts here.

Posted in Universal MosSCI sites
3 comments on “Verification of insertions into universal mosSCI sites
  1. Dhaval says:

    Hi Christian,

    Thanks for posting the annotated ApE files for these sites. Looks like the OxTi185 file is missing the primer sequences.



    • Christian Frøkjær-Jensen says:

      Hi Dhaval,
      Sorry for the slow reply – I’ve been traveling. When I download the oxTi185 file it does contain the oligos. Will you let me know if you still have trouble with it and I’ll send it to you directly. Cheers, Christian

      • Dhaval says:

        Hi Christian,

        I downloaded the file again and everything was there. I must have messed up the file the first time round.



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