We have released the miniMos reagents prior to publication so that other researchers can use them without having to wait.
The plasmids can be ordered either individually (see plasmids) or as a kit (29 plasmids – 8 co-injection vectors, 16 cloning vectors, 3 positive controls and 2 LacO vectors) from Addgene. Please see this page for information about ordering the kit. If you plan to order more than 7 plasmids it is cheaper to order the full kit. Addgene is a non-profit plasmid repository and we do not receive any compensation or commission – it is just the easiest way to distribute plasmids and saves us a lot of time dealing with shipping and MTAs.
Also, several people in the lab are in the process of developing a gene-trap system based on the miniMos transposon. Please don’t use the miniMos reagents prior to publication to compete with these efforts. We will release the gene-trap reagents as soon as possible.
Please let me know if you have any problems with the reagents – or if you notice mistakes in the protocols or plasmid files – so I can fix them as soon as possible. Using the feedback option is easiest.
Please cite “C. Frøkjær-Jensen and E.M. Jorgensen, unpublished reagents” if you manage to use the reagents in a publication before we publish the method.