LacO insertions

Use search box to limit strains to chromosomes (LG1, LG2, LG3, LG4, LG5 or LGX) or resistance marker (PuroR, NeoR).

Each strain has been tested for antibiotic resistance but not for LacI binding. Please note that the lacO insertions were put into a strain with the syIs46 insertion. This insertion has both LacI-GFP but also lacO sites. The brightest spot present in these strains are therefore from the syIs46 array integration and the dimmer spot from the 256x lacO miniMos insertion (thank you to Peter Meister for clarifying this). It is highly recommended to outcross 256x lacO from syIs46 for use as a chromosomal marker.

Please request strains from the CGC.
Strain column has link to the strain info at the CGC.

If you find the strains useful then please cite “Frøkjær-Jensen C, Davis MW, Sarov M, Taylor J, Flibotte S, LaBella M, Pozniakovski A, Moerman DG and Jorgensen EM. Random and targeted transgene insertion in C. elegans using a modified Mos1 transposon. Nature Methods. (In press)”.

Chr.Genetic pos.Position (WB230)CargoAlleleStrainInsertion marker(s)GenotypeFlanking sequenceComments
LG3III:-26.98118,056256x LacO and NeoRoxTi467EG7522cb-unc-119(+) and NeoRsyIs46 II; unc-119(ed3) III oxTi467 IIITACATCATAGCCTGATCCT
LG1I:-19.15147,105256x LacO and NeoRoxTi468EG7523cb-unc-119(+) and NeoRoxTi468 I ; syIs46 II; unc-119(ed3) IIITATCTTTGTCACTCAGACGTTCCACAAGTT
LG4IV:14.3815,809,942256x LacO and NeoRoxTi469EG7524cb-unc-119(+) and NeoRsyIs46 II; unc-119(ed3) III ; oxTi469 IVTAAGATTTAATTGTTAAAAAAAAGCCCTAA
LG2II:-0.396,267,597256x LacO and PuroRoxTi470EG7525cb-unc-119(+) and PuroRoxTi470 II syIs46 II; unc-119(ed3) IIITATTTGTCAACTGAAATTTATCATTTTTTA
LG2II:10.5912,635,479256x LacO and PuroRoxTi471EG7526cb-unc-119(+) and PuroRoxTi471 II ; syIs46 II; unc-119(ed3) IIITAGAACTTCAAGACGCGAACCTTGAAAAAA
LG2II:24.1115,142,879256x LacO and PuroRoxTi472EG7527cb-unc-119(+) and PuroRoxTi472 II ; syIs46 II; unc-119(ed3) IIITAATGTATTTTAATACAGAATAGTCTCGAG
LG2II:-0.586,074,479256x LacO and PuroRoxTi473EG7528cb-unc-119(+) and PuroRoxTi473 II ; syIs46 II; unc-119(ed3) IIITAATATGACAATTAGTAGTCATATGGATGC
LG5V:24.9720,209,942256x LacO and PuroRoxTi474EG7529cb-unc-119(+) and PuroRsyIs46 II ; unc-119(ed3) III ; oxTi474 IVTAAATGATTGAACAGATTCTTTGTTTTGAA
LG5V:-19.851,245,918256x LacO and PuroRoxTi475EG7530cb-unc-119(+) and PuroRsyIs46 II; unc-119(ed3) III ; oxTi475 VTATATGTACATATTATTCTCATAAATCTCA
LG5V:2.8310,899,001256x LacO and PuroRoxTi476EG7531cb-unc-119(+) and PuroRsyIs46 II; unc-119(ed3) III ; oxTi476 VTATTACTTTTCTAAAGTTTTCAAGAATAAA
LG4IV:3.598,033,901256x LacO and PuroRoxTi477EG7532cb-unc-119(+) and PuroRsyIs46 II; unc-119(ed3) III ; oxTi477 IVTACATAAAAAATACTCATTGATTAATTGGT
LG5V:4.6212,728,334256x LacO and PuroRoxTi478EG7533cb-unc-119(+) and PuroRsyIs46 II; unc-119(ed3) III ; oxTi478 VTAATACAAAAAATTCGATATTTTACTCAAA
LG1I:-21.834,915256x LacO and PuroRoxTi479EG7541cb-unc-119(+) and PuroRoxTi479 I ; syIs46 II; unc-119(ed3) IIITACTACACGTGGGCAAGTTTATACAGTTTT
LG2II:-5.644,063,707256x LacO and PuroRoxTi480EG7534cb-unc-119(+) and PuroRoxTi480 II syIs46 II; unc-119(ed3) IIITATCTTATCGTATGAATTGTGTCCGTTTCT
LG1I:2.107,530,607256x LacO and PuroRoxTi481EG7535cb-unc-119(+) and PuroRoxTi481 I ; syIs46 II; unc-119(ed3) IIITAATTGTTGGAGCAATTGTTGTCCTTGGTT
LG4IV:4.039,080,905256x LacO and PuroRoxTi482EG7536cb-unc-119(+) and PuroRsyIs46 II; unc-119(ed3) III ; oxTi482 IVTAAATAATTTGATTCTTTTAAGACAATTAT
LGXX:7.0412,405,642256x LacO and PuroRoxTi483EG7477cb-unc-119(+) and PuroRsyIs46 II; unc-119(ed3) III ; oxTi483 XTATCCAGCCCGACGAGTCTAGTAGTGGATA
LG3III:3.9610,613,761256x LacO and PuroRoxTi485EG7538cb-unc-119(+) and PuroRsyIs46 II; unc-119(ed3) III Ti485 IIITATATCAATTGGGAATGCACGGAAATTTAA
LG5V:-17.211,810,279256x LacO and PuroRoxTi486EG7539cb-unc-119(+) and PuroRsyIs46 II; unc-119(ed3) III ; oxTi486 VTATGTATTAAGTTGGCCTATATACCAAGTT
LGXX:21.8415,091,074256x LacO and PuroRoxTi487EG7478cb-unc-119(+) and PuroRsyIs46 II ; unc-119(ed3) III ; oxTi487 XTAGTGAAAGAAACGCTAGGAGAAGATACTG