A graduate student in Erik’s lab (Matt LaBella) has built two mapping strains based on fluorescent miniMos insertions. Each strain contains three distinct fluorescent markers on separate chromosomes in a him background. He and others in the lab has used them successfully to map new mutations to a chromosome before whole-genome sequencing. We’ve deposited the mapping strains EG8040 and EG8041 at the CGC if you want to give them a try.
See here for more information about the markers.