Blog Archives

Fluorescent mapping strains

A graduate student in Erik’s lab (Matt LaBella) has built two mapping strains based on fluorescent miniMos insertions. Each strain contains three distinct fluorescent markers on separate chromosomes in a him background. He and others in the lab has used

Posted in miniMos

Verification of insertions into universal mosSCI sites

I’ve had a few people ask me for sequences surrounding the universal mosSCI sites and oligos that can be used to verify insertions and homozygosity of the inserts. I have posted ApE files with the local genomic environment and the

Posted in Universal MosSCI sites

DNA quality and MosSCI insertion frequency

Morris Maduro has previously pointed out that the quality of the DNA preparation for injection made a large difference in the number of rescued F1 progeny (see Worm Breeders Gazette). Since we had started seeing some variability in the insertion

Posted in Improvements

Problems with pCFJ1324 and pCFJ1319 LacO vectors from Addgene kit

updated 10-12-2013 I have re-transformed the plasmids into EPI400 cells and verified by digest that the LacO repeats are intact in the original version of the plasmids. The plasmid was resubmitted to Addgene 9-26-2013 and the fully intact plasmid should

Posted in Reagent problems

miniMos reagents available from Addgene

We have released the miniMos reagents prior to publication so that other researchers can use them without having to wait. The plasmids can be ordered either individually (see plasmids) or as a kit (29 plasmids – 8 co-injection vectors, 16

Posted in miniMos

miniMos reagents

We are planning to release the miniMos reagents through Addgene. They’ve promised us that they should be ready for distrubtion by the end of July. I’ll post information here – as well as detailed protocols for insertion and for mapping

Posted in miniMos

New site

Hello and welcome! I decided to update the old site describing mos1-mediated techniques to modify the C.elegans genome. I developed the techniques together with others in the Jorgensen laboratory (mainly Wayne Davis) and several collaborators outside the lab. I hope

Posted in General News